Composite

Part:BBa_K611059:Design

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-27)


pTet Promoter Variant #9 in Characterization Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).


Source

Mutagenic PCR of the Tet repressible promoter, R0040, and standard BioBrick assembly of parts from the registry.

References